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Figure 5 | BMC Veterinary Research

Figure 5

From: In vitro prion protein conversion suggests risk of bighorn sheep (Ovis canadensis) to transmissible spongiform encephalopathies

Figure 5

Conversion efficiency ratio (CER) assay using bighorn sheep (BHS) and native host species substrates. Immunoblots to assess proteinase K-resistant prion protein in BHS CER assay substrate prepared at either pH 7.4 or 3.5 and incubated with (A) domestic sheep classical scrapie, (B) transmissible mink encephalopathy (TME) agent, (C) white-tailed deer (WTD) chronic wasting disease (CWD) agent or (D) wapiti CWD agent. Domestic sheep, mink or white-tailed deer substrates were used as controls in A, B or C &D, respectively. Lanes labeled “none” contained an equal amount of the indicated TSE agent but no substrate. Raw densitometric values for each sample are displayed below each lane. Immunoblots used monoclonal antibody BAR 224.

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