Species | Assay | Primer sequence (5′ → 3′) | Annealing | Product size |
---|---|---|---|---|
Oligonucleotides used for screening (Group I) a | ||||
B. bigemina | PCR | F-CATCTAATTTCTCTCCATACCCCTCC | 55°C | 278 bp |
R-CCTCGGCTTCAACTCTGATGCCAAAG | ||||
nPCR | F-CGCAAGCCCAGCACGCCCCGGTGC | 55°C | 170 bp | |
R-CCGACCTGGATAGGCTGTGTGATG | ||||
B. bovis | PCR | F-CACGAGGAAGGAACTACCGATGTTGA | 55°C | 360 bp |
R-CCAAGGAGCTTCAACGTACGAGGTCA | ||||
nPCR | F-TCAACAAGGTACTCTATATGGCTACC | 55°C | 298 bp | |
R-CTACCGAGCAGAACCTTCTTCACCAT | ||||
Oligonucleotides used for phylogenetic study (Group II) b | ||||
B. bigemina | PCR | F-GTGCTGCTTAATCGCACAAAC | 55°C | 963 bp |
R-AAGATGCCTTCTTCGGTGATG | ||||
nPCR | F-CGGATCCTGTTATCGTTCCTG | 56°C | 853 bp | |
R-GAAGTTACGCCTGGAGTTGG | ||||
B. bovis | PCR | F-TCAGATTGTTCAAAGAGAGTGCATCC | 55°C | 1280 bp |
R-GTCTTCACCGTTGGAAGTAGTTGAGTC | ||||
nPCR | F-CACGAGGAAGGAACTACCGATGTTGA | 64°C | 1009 bp | |
R-CCTTTGTAGGTTGGCCAACAGTTTCG |