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Table 1 Primary and nested PCR primers used for PCR amplifications

From: Molecular diagnosis and phylogenetic analysis of Babesia bigemina and Babesia bovishemoparasites from cattle in South Africa

Species

Assay

Primer sequence (5′ → 3′)

Annealing

Product size

Oligonucleotides used for screening (Group I) a

B. bigemina

PCR

F-CATCTAATTTCTCTCCATACCCCTCC

55°C

278 bp

R-CCTCGGCTTCAACTCTGATGCCAAAG

nPCR

F-CGCAAGCCCAGCACGCCCCGGTGC

55°C

170 bp

R-CCGACCTGGATAGGCTGTGTGATG

B. bovis

PCR

F-CACGAGGAAGGAACTACCGATGTTGA

55°C

360 bp

R-CCAAGGAGCTTCAACGTACGAGGTCA

nPCR

F-TCAACAAGGTACTCTATATGGCTACC

55°C

298 bp

R-CTACCGAGCAGAACCTTCTTCACCAT

Oligonucleotides used for phylogenetic study (Group II) b

B. bigemina

PCR

F-GTGCTGCTTAATCGCACAAAC

55°C

963 bp

R-AAGATGCCTTCTTCGGTGATG

nPCR

F-CGGATCCTGTTATCGTTCCTG

56°C

853 bp

R-GAAGTTACGCCTGGAGTTGG

B. bovis

PCR

F-TCAGATTGTTCAAAGAGAGTGCATCC

55°C

1280 bp

R-GTCTTCACCGTTGGAAGTAGTTGAGTC

nPCR

F-CACGAGGAAGGAACTACCGATGTTGA

64°C

1009 bp

  

R-CCTTTGTAGGTTGGCCAACAGTTTCG

  
  1. a Group I oligonucleotide sequences were taken from published work [8].
  2. b Group II primers were designed in this study.