Evaluation of immune and stress status in harbour porpoises (Phocoena phocoena): can hormones and mRNA expression levels serve as indicators to assess stress?

Table 1 Primer-specific sequences, annealing temperatures and base pair length for housekeeping genes, cytokines, acute-phase proteins and heat shock protein

Cellmediators/mol. biomarkers		Primer sequences (5′–3′)	Annealing temperature	Base pair length
YWHAZ	S	AGA CGG AAG GTG CTG AGA AA	58°C	208
	AS	TCA TVA CCA GCA GCA ACT TC
GAPDH	S	GCC AAA AGG GTC ATC ATC TC	57°C	201
	AS	GGG GCC ATC CAC AGT CTT CT
IL-10	S	CCT GGG TTG CCA AGC CCT GTC	62°C	208
	AS	ATG CGC TCT TCA CCT GCT CC
TNFα	S	GGC TGA ACA CAT ATG CCA AC	57°C	111
	AS	TGA AGA GGA CCT GGG AGT AGA
HP	S	CTG GCA GGC TAA GAT GGT TT	54°C	75
	AS	GTC AGC AGC CAT TGT TCA TT
CRP	S	TTC TCG TAT GCC ACC AAG AG	54°C	192
	AS	TTC AGA CCC ACC CAC TGT AA
HSP 70	S	GGG GCT GAA CGT GCT GAG G	62°C	280
	AS	CCG CTT GTT CTG GCT GAT GTC

ISSN: 1746-6148