The microsporidian Enterocytozoon hepatopenaei is not the cause of white feces syndrome in whiteleg shrimp Penaeus (Litopenaeus) vannamei

Table 1 Primer sequences used

Primer name	Sequence (5′→ 3′)	Size of amplicon (bp)
MF1	CCG GAG AGG GAG CCT GAGA	951
MR1	GAC GGG CGG TGT GTA CAA A
1 ^st PCR step		779
ENF779	CAG CAG GCG CGA AAA TTG TCC A
ENR779	AAG AGA TAT TGT ATT GCG CTT GCT G
Nested PCR step		176
ENF176	CAA CGC GGG AAA ACT TAC CA
ENR176	ACC TGT TAT TGC CTT CTC CCT CC
DIG microsporidian probe		411
ENF411	AGG TGG TGT TAA AAG CCA TTG AG
ENR176	TAC CTC ATG TCT CCA ATC TAC GAT A
DIG GFP probe		513
GFPF513	TTC ATC TGC ACC ACC GGC AAC CTG
GFPR513	CTG GTA GTG GTC GGC GAG CTG CAC

ISSN: 1746-6148