Figure 5
Adipogenic differentiation of bMSC isolated from fetal bone marrow. (A) Culture of bMSC under adipogenic conditions induced drastic morphology changes including formation of cell aggregates and accumulation of lipid vacuoles in the cytoplasm. (B) AP2 mRNA levels increased (P < 0.05) in differentiating bMSC at Days 12 and 18 of culture. (C) In contrast, expression levels of PPARγ mRNA were similar between treatments. (D) PPARγ was immunodetected in differentiated bMSC but not in controls at Day 18 of culture. (E) Adipogenesis was confirmed by oil red (OR) staining of lipids vacuoles in differentiated bMSC at Day 18 of culture. Phase contrast, PPARγ and merged with DAPI for control and differentiation bMSC. Superscripts (a, b,*) represent significant (P < 0.05) differences between treatment and sampling days. Bar scale: (A) 500 μm; (D) 20 μm; (E) 500 μm.