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Table 3 Observation of activities of Ancistrocladus uncinatus extracts and fractions on haemadsorbing African swine fever virus in-vitro 5-days post infection

From: Phytochemical analysis and in-vitro anti-African swine fever virus activity of extracts and fractions of Ancistrocladus uncinatus, Hutch and Dalziel (Ancistrocladaceae)

 

1

2

3

4

5

6

7

8

9

10

11

12

Mg/ml

ASF NIG/99 (+)

Acetone (C)

Hexane(C)

DCM (C)

Chloroform (C)

Methanol (C)

Hexane (F)

Methanol (F)

Ethyl acetate (F)

Chloroform (F)

Blank

Placebo (−)

1

+++

-

+

-

++

-

-

-

-

+

 

-

0.5

+++

-

++

-

++

-

-

-

-

+

 

-

0.25

+++

-

++

-

++

-

-

-

-

++

 

-

0.125

+++

-

+++

-

+++

-

-

-

+

++

 

-

0.0625

+++

-

+++

+

+++

-

-

+

+

+++

 

-

0.03125

+++

+

+++

++

+++

++

+

++

++

+++

 

-

0.015625

+++

++

+++

++

+++

++

++

++

++

+++

 

-

0.0078125

+++

+++

+++

+++

+++

+++

+++

+++

+++

+++

 

-

  1. +++ = ≥50 rosettes were observed and counted in the well; ++ = 10–50 rosettes were observed and counted; + = ≤10 rosettes were observed and counted. - = no rosette was observed. C = crude extract and F = fraction of extract. N.B. Rosette formation is an indication of virus infection of the macrophage cells in the PBMC medium.
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BMC Veterinary Research

ISSN: 1746-6148

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