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Table 2 Summary of observations for selected surface proteins

From: Comparative analysis of the surface exposed proteome of two canine osteosarcoma cell lines and normal canine osteoblasts

Protein name

Mass spec

qRT-PCR

Western blot

ICC

Thrombospondin-1

    

CnOb

D

High

High

N/A

POS

ND

Low

Mid

N/A

HMPOS

ND

Low

Low

N/A

CD44

    

CnOb

ND

High

Low

Low

POS

D

Low

High

High

HMPOS

D

Low

Mid

High

CYR61

    

CnOb

D

High

High

N/A

POS

D

Low

High

N/A

HMPOS

D

Low

Low

N/A

Plexin B2

    

CnOb

ND

Low

N/A

N/A

POS

D

High

N/A

N/A

HMPOS

D

Mid

N/A

N/A

Notch-2

    

CnOb

ND

Low

N/A

N/A

POS

D

Mid

N/A

N/A

HMPOS

D

High

N/A

N/A

  1. A summary of observations for a subset of the surface-exposed proteins identified by mass spectrometry in cultured normal canine osteoblasts (CnOb) and two validated canine osteosarcoma cell lines (POS and HMPOS) and the results of their evaluation via secondary techniques (qRT-PCR, Western blot, and immunocytochemistry [ICC]).
  2. D Detected.
  3. ND Not detected.
  4. High, Mid, Low = relative expression levels observed.