Isothermal loop-mediated amplification (lamp) for diagnosis of contagious bovine pleuro-pneumonia

BMC Veterinary Research

Table 4 Experimental samples from Cameroun tested for M. mycoides subsp. mycoides (Mmm) by different methods and LAMP; all samples were collected >45 days post intubation and showed specific pathology on post mortem PM except 8410

Origin	Specimen ID	PCR	cELISA	Culture	LAMP
Mmm inoculated cow, 8429	Serum	nd	+	nd	+
Mmm inoculated cow, 8429	Pleural fluid	+	nd	+	+
Mmm inoculated cow, 8462	Serum	nd	+	nd	+
Mmm inoculated cow, 8462	Pleural fluid	+	nd	+	+
Mmm inoculated cow, 05	Serum	nd	+	nd	+
	Lung	+	nd	+	+
	Pleural fluid	+	nd	+	+
Bov. before inoculation; 8178	Serum	-	nd	nd	-
Mmm inoculated cow, 8178	Serum	nd	+	nd	+
Mmm inoculated cow, 07	Lung	+	nd	+	+
Mmm inoculated cow, 07	Pleural fluid	+	nd	+	+
Mmm inoculated cow, 08	Lung	+	nd	+	+
Mmm inoculated cow, 08	Pleural fluid	+	nd	+	+
Mmm inoculated cow, 8454	Serum	nd	+	nd	+
Mmm inoculated cow, 8454	Serum	nd	+	nd	+
Mmm inoculated cow, 49	Lung	+	nd	+	+
Mmm inoculated cow, 50	Lung	+	nd	+	+
Mmm inoculated cow, 56	Pleural fluid	+	nd	+	+
Mmm inoculated cow, 8410	Lung	-	nd	nd	-
Mmm inoculated cow, 8410	Pulmon. lymph node	-	nd	nd	-

ISSN: 1746-6148