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Table 3 Detection of M. mycoides subsp . mycoides in bronchial lavage fluid of cattle that were experimentally infected by contact with animals that were directly infected 4 weeks before with M. mycoides subsp . mycoides a) strain Afadé or b) strain L2 (Miserez et al., [[15]])

From: Isothermal loop-mediated amplification (lamp) for diagnosis of contagious bovine pleuro-pneumonia

Days before/after contact infection

Viable M. mycoides subsp. mycoides / ml

Nested PCR*

LAMP

- 21

 

-

-

+ 16

 

-

-

+ 21

 

-

-

+ 29

 

-

-

+ 35

 

-

-

+ 42

 

-

-

+ 49

 

-

-

+ 56

 

-

-

+ 64

 

-

-

+ 70

 

-

+

+ 77

1.0 × 102

+

+

+ 84

 

+

+

+ 92

2.5 × 102

+

+

+ 100

3.5 × 102

+

+

+ 105

8.5 × 102

+

+

+ 112

1.0 × 102

+

+

+ 119

1.0 × 102

+

+

+ 125

1.0 × 102

+

+

+ 132

 

+

+

+ 140

 

-

+

+ 146

 

-

+

+ 153

 

+

+

+ 160

 

+

+

+ 168

 

-

+

+ 175

 

+

+

+ 181

 

+

+

+ 188

1.0 × 102

+

+

+ 195

1.0 × 102

+

+

+ 202

 

-

-

+ 209

 

-

-

+ 216

 

-

+

+ 223

 

-

-

  1. * nested PCR results [15].