Molecular cloning and expression analysis of peptidase genes in the fish-pathogenic scuticociliate Miamiensis avidus

Table 2 List of primers used in quantitative reverse-transcription PCR (RT-PCR)

Target clone/gene	Primers	Sequences (5^′- 3^′)	Product size (bp)
MaPro 1	Forward	TGCTTCCACTTCAGTTTTATCAGTCG	266
	Reverse	GGTTAAGTTCAACTGTGGGGATTTCTAA
MaPro 2	Forward	TCTTGAGAGCTTCTGCTGCCAC	271
	Reverse	TCTTGGATGTTTAATTCGGTGCTGT
MaPro 3	Forward	AATCCAACGAAGACATCAGAATCTTCT	246
	Reverse	CAGGGACTTATCTGGAAGGTCTGGA
MaPro 4	Forward	TAGCTTCAATTGCTTCTGGTAGTCTTG	277
	Reverse	ATCCATGTTTATTCCACATAGTCCATTAC
MaPro 5	Forward	ATTTCAAGCGATTGGAAGCTAAGAATC	308
	Reverse	AATAATCCCAAATAGAATATTACCCATCTTC
MaPro 6	Forward	ACTTGGACTGCTGGATACAACAAAC	310
	Reverse	CGGTGGAGATTCTGGTTTGAAC
MaPro 7	Forward	CGGTGGAGATTCTGGTTTGAAC	212
	Reverse	TTCGGCATCAACAGAATGGTAGATAC
MaPro 8	Forward	ACGTTTTATTAGAAAGCCAAGGTAACC	260
	Reverse	GGTATTTTCGTCGGTGTATTTGTAGCT
MaPro 9	Forward	GGGAAAAGGAAACTCTGCATTCG	348
	Reverse	CATCCATTTTCAGCAGTGTACAGTTCTAT
MaPro 10	Forward	CAGATAATGGCTCTACTAATATTGCACTC	243
	Reverse	AGGGATCTTCACTTCCATTTGTGAATAC
MaPro 11	Forward	ATTTGGCTCAATGGAGGACCTG	217
	Reverse	CAGCGGTATTATCATCAGTGTAAGAGT
MaPro 12	Forward	CCACCTACAAACCCCAAGGAGAC	268
	Reverse	GCGAAAGAGTTGTCTTCCCAGACT
MaPro 13	Forward	CAGCTCTTTTAACAGAAGGAGCTACAC	352
	Reverse	TTTCTGAACTTCGGTATCCACCATA
MaPro 14	Forward	AGGGTATCTTCGAACAGCTCTTCG	319
	Reverse	CATTGGGACAAGAGACTGAACAGTC
MaPro 15	Forward	AGCCTTGGAATGGAAATACTTTCGCTG	319
	Reverse	CCAACACAGTATCCGTTAGAGCTACAG
MaPro 16	Forward	ACTCACGGATAGAACAATGCTCTTGC	314
	Reverse	TTAAAGTGCTTGCGAGCCACTTCC
MaPro 17	Forward	TTGCAAGTTTCCTCTGGATTTGAATC	327
	Reverse	TCCGTATATAAGTTCAATTGTGGCATC
β-tubulin	Forward	GTATGATCATTGATAACGAAGCCCTCTACG	323
	Reverse	TCTGGGATCGGCGGCGCACATCATG

ISSN: 1746-6148