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Table 2 Cell viability of canine cruciate ligamentocytes in response to different inhibitors and SNP treatment

From: Nitric oxide induces cell death in canine cruciate ligament cells by activation of tyrosine kinase and reactive oxygen species

Inhibitor pretreatment

CCL cell viability (n = 8)

CaCL cell viability (n = 8)

  

SNP treatment

SNP treatment

  

-

+

-

+

zVAD.fmk

0

100 ± 6.0

75.5 ± 3.1

100 ± 5.9

73.6 ± 2.4

 

100 μM

92.5 ± 5.3

84.5 ± 4.3

92.2 ± 6.3

88.2 ± 3.6*

CalC

0

100 ± 11.3

79.9 ± 5.3

100 ± 5.9

78.2 ± 7.2

 

25 nM

93.5 ± 6.9

73.4 ± 3.3

100 ± 6.0

68.7 ± 7.0

PD98059

0

100 ± 5.6

75.8 ±6.4

100 ± 4.1

74.7 ± 9. 3

 

10 μM

81.2 ± 7.2*

61.8 ± 3.1*

78.3 ± 7.2*

52.2 ± 9.2*

SB202190

0

100 ±11.3

73.4 ± 5.4

100 ± 6.0

74.2 ± 7.7

 

10 μM

74.2 ±2.1*

65.9 ± 4.5

75.2 ± 5.8*

79.8 ± 2.4

Genistein

0

100 ± 6.0

69.6 ± 6.1

100 ± 5.8

72.3 ± 5.7

 

50 μM

101 ± 6.4

97.4 ±9.3**

106.1 ±6.3

101.9 ± 2.5**

NS-398

0

100 ± 5.4

64.9 ± 3.6

100 ± 5.4

63.2 ± 4.1

 

50 μM

89.5 ± 17.7

78.1 ± 3.9*

88.5 ± 4.7*

88.8 ± 2.5**

 

100 μM

74.7 ± 14.4

75.3 ± 4.2*

81.8 ± 12.1

92.4 ± 2.7**

SN-50

0

100 ± 8.3

74.6 ± 3.8

100 ± 8.4

81.2 ± 2.4

 

50 μM

97.3 ± 8.6

64.4 ± 12.6

101.4 ± 4.5

67.3 ± 2.7*

PDTC

0

100 ± 8.3

74.6 ± 3.8

100 ± 8.4

81.2 ± 2.4

 

10 μM

42.7 ± 7.7**

5.5 ± 1.9**

43.8 ± 2.3**

2.1 ± 1.7**

Uric acid

0

100 ± 9.2

73.5 ± 5.4

100 ± 5.6

78.2 ± 2.4

 

0.5 mM

103.6 ± 9.5

102.7 ± 12.1*

101.9 ± 6.2

94.6 ± 5.9*

PTIO

0

100 ± 6.2

64.9 ± 2.6

100 ± 9.3

61.2 ± 4.6

 

5 μM

87.9 ± 7.3

80.9 ± 1.5*

96.2 ± 9.6

73.8 ± 3.2*

Taxifolin

0

100 ± 3.7

76.2 ± 4.2

100 ± 5.6

74.3 ± 7.7

 

100 μM

91.6 ± 11.5

84.8 ± 10.6

101.3 ± 4.1

97.3 ± 6.8*

  1. Values correspond to the mean ± SD, calculated by using the formula from M&M section of three separate experiments of n different cell donors, each performed in triplicates. Cruciate ligamentocytes were preincubated with the indicated concentrations of the inhibitors for 2 h. SNP or none SNP were then added to the cultures and allowed to incubate for an additional 18-h period. SNP concentrations used were 0.2 to 0.25 mM. Cell viability was assayed by MTT assay. P-values indicate difference within the same type of cell and SNP treatment versus absent inhibitor: *, P < 0.05; **, P < 0.01.