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Table 5 Estimates (95% lower, upper confidence levels) of standard deviation (SDr) and relative standard deviation (RSDr) of repeatability of uniplex and mRT-qPCR assays

From: One-step multiplex real time RT-PCR for the detection of bovine respiratory syncytial virus, bovine herpesvirus 1 and bovine parainfluenza virus 3

Virus

Uniplex

Multiplex

 

SDr

RSDr (%)

SDr

RSDr (%)

BoHV-1

0.39

(0.31, 0.49)

1.04

(0.82, 1.31)

1.15

(0.94, 1.41)

3.10

(2.53, 3.79)

BRSV

0.46

(0.36, 0.59)

1.26

(0.99, 1.60)

1.30

(1.10, 1.53)

3.40

(2.88, 4.02)

BPI3-i

0.58

(0.29, 1.17)

1.50

(0.75, 3.00)

0.70

(0.59, 0.83)

1.79

(1.50, 2.13)

BPI3-ii

0.57

(0.43, 0.75)

1.36

(1.03, 1.80)

0.86

(0.71, 1.04)

2.07

(1.71, 2.50)