Initial media testing | Basic media | Supplementation |
---|---|---|
DMEM +10% FCS | - | |
DMEM/Ham's F-12 (1:1) +10% FCS | - | |
Ham's F-12 +10% FCS | - | |
McCoys-5A +10% FCS | - | |
Medium 199 +10% FCS | - | |
Media conditioning and additives | Basic media | Supplementation |
Ham's F-12 +10% FCS | - | |
Ham's F-12 +10% FCS, cond. | - | |
Ham's F-12 +10% FCS, cond. | + EGF | |
Ham's F-12 +10% FCS, cond. | + insulin | |
Ham's F-12 +10% FCS, cond. | + hydrocortisone | |
Ham's F-12 +10% FCS, cond. | + EGF, insulin | |
Ham's F-12 +10% FCS, cond. | + insulin, hydrocortisone | |
Ham's F-12 +10% FCS, cond. | + EGF, hydrocortisone | |
Ham's F-12 +10% FCS, cond. | + EGF, insulin, hydrocortisone | |
Differentiated cell culture | Lower compartement | Upper compartment |
Ham's F-12 +10%FCS, cond., EGF, insulin | a) Ham's F-12 +10%FCS, cond., EGF, insulin | |
b) no medium (air-liquid interface) |