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Table 3 Media composition in the different steps of cell culture optimization

From: Establishment and characterization of a differentiated epithelial cell culture model derived from the porcine cervix uteri

Initial media testing

Basic media

Supplementation

 

DMEM +10% FCS

-

 

DMEM/Ham's F-12 (1:1) +10% FCS

-

 

Ham's F-12 +10% FCS

-

 

McCoys-5A +10% FCS

-

 

Medium 199 +10% FCS

-

Media conditioning and additives

Basic media

Supplementation

 

Ham's F-12 +10% FCS

-

 

Ham's F-12 +10% FCS, cond.

-

 

Ham's F-12 +10% FCS, cond.

+ EGF

 

Ham's F-12 +10% FCS, cond.

+ insulin

 

Ham's F-12 +10% FCS, cond.

+ hydrocortisone

 

Ham's F-12 +10% FCS, cond.

+ EGF, insulin

 

Ham's F-12 +10% FCS, cond.

+ insulin, hydrocortisone

 

Ham's F-12 +10% FCS, cond.

+ EGF, hydrocortisone

 

Ham's F-12 +10% FCS, cond.

+ EGF, insulin, hydrocortisone

Differentiated cell culture

Lower compartement

Upper compartment

 

Ham's F-12 +10%FCS, cond., EGF, insulin

a) Ham's F-12 +10%FCS, cond., EGF, insulin

  

b) no medium (air-liquid interface)

  1. DMEM Dulbecco's MEM medium, FCS fetal calf serum, cond. conditioned. Concentration of the supplements: 10 ng/mL murine EGF, 1 μg/mL porcine insulin, 0.5 μg/mL hydrocortisone. All growth media were supplemented with 100 U/mL penicillin/100 μg/mL streptomycin, 50 μg/mL gentamycin, 1 μg/mL amphotericin B, 10 μg/mL reduced glutathione and 10 μg/mL ascorbic acid.