Figure 4From: Establishment and characterization of a differentiated epithelial cell culture model derived from the porcine cervix uteriCervical epithelial cells of three animals were cultured for 24 h, 48 h and 72 h in different growth medium compositions. Viability and proliferation were measured in triplicates using the Roche WST-1 Proliferation Assay. Extinctions of the media were measured at 450 nm after 30 min incubation with the WST-1 reagent. A) Ham's F-12 relative to the unconditioned medium, and B) conditioned Ham's F-12 with different additives relative to the basic conditioned Ham's F-12.Back to article page