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Table 1 Cell surface, adipogenic, osteogenic, chondrogenic and neurogenic markers analysed by RT-qPCR

From: Isolation and characterization of ovine mesenchymal stem cells derived from peripheral blood

Genes

Accession number

Primer sequences

Amplicon size (bp)

 

Forward (5’ → 3’)

Reverse (5’ → 3’)

 

Cell Surface Markers

   

CD29

AF349461

GTGCCCGAGCCTTCAATAAAG

CCCGATTTTCAACCTTGGTAATG

87

CD34

AB021662

TGGGCATCGAGGACATCTCT

GATCAAGATGGCCAGCAGGAT

107

CD45

NM_001206523

CCTGGACACCACCTCAAAGCT

TCCGTCCTGGGTTTTATCCTG

101

CD73

BC114093

TGGTCCAGGCCTATGCTTTTG

GGGATGCTGCTGTTGAGAAGAA

115

CD90

BC104530

CAGAATACAGCTCCCGAACCAA

CACGTGTAGATCCCCTCATCCTT

96

CD105

NM_001076397

CGGACAGTGACCGTGAAGTTG

TGTTGTGGTTGGCCTCGATTA

115

Differentiation Markers

   

PPARG

NM_001100921

GCCCTGGCAAAGCATTTGTA

TGTCTGTCGTCTTTCCCGTCA

94

SCD 1

AJ001048

CCCAGCTGTCAGAGAAAAGG

GATGAAGCACAACAGCAGGA

115

IL6

FJ409227.1

CAGCAAGGAGACACTGGCAG

TGATCAAGCAAATCGCCTGAT

101

COL1A1

AF129287

CCTGCGTACAGAACGGCCT

ACAGCACGTTGCCGTTGTC

93

BGLAP

DQ418490

CCCAGGAGGGAGGTGTGTG

CTAGACCGGGCCGTAGAAGC

99

BGN

NM_001009201.1

AACATGAACTGCATTGAGATGGG

GCGAAGGTAGTTGAGCTTCAGG

93

LUM

NM_173934.1

AAGCAATTGAAGAAGCTGCACA

TTAGTGAGCTGCAGGTCCACC

92

NES

194665083

CAAATCGCCCAGGTCCTG

GCCTCTAGGAGGGTCCTGTATGT

95

NEFM

194669578

GCTCGTCATCTGCGAGAATACC

CACCCTCCAGGAGTTTCCTGTA

91

NELF

27806522

CGCTATGCAGGACACAATCAAC

GGGTCTCCTCACCTTCCAAGA

161

TUBB3

116004470

GACCTCGAGCCTGGAACCAT

GCCCCACTCTGACCAAAGATG

92

MAP2

194664873

TGTCCCAGTGGAGGAAGGTTT

TCTTGTCTAGTGGCTCGGCTG

95

PRNP

BC119821

CGCAGAAGCAGGACTTCTGAA

TGGATTTGTGTCTCTGGGAAGA

86

Housekeeping genes

G6PDH 2

AJ507200

TGACCTATGGCAACCGATACAA

CCGCAAAAGACATCCAGGAT

76

HPRT 3

EF078978

AGGTGTTTATTCCTCATGGAGTAATTATG

GGCCTCCCATCTCCTTCATC

79

  1. GenBank accession numbers of the sequences used for primer design. Primer sequences (F: Forward and R: Reverse) and the length of the amplicon in base pairs (bp).
  2. 1 Primers described in Dervishi et al. [60].
  3. 2 Primers described in García-Crespo et al. 2005 [61].
  4. 3 Primers described in Lyahyai et al. 2010 [62].