Figure 3

E ₂ -induced SBD-1 expression in ovine oviduct epithelial cells via cAMP/PKA pathway, PKC pathway and NF-κB pathway. Cells treated with the PKA (H-89, 50 μM), the PKC (H-7, 50 μM), and NF-κB inhibitors (PDTC, 50 μM) showed significantly lower SBD-1 expression compared to the control. Furthermore, a blank control (water substituted for cDNA) was included in each qPCR reaction mixture, and a control was set up containing water instead of E₂ or/and inhibitor(s) in every group. Total RNA extracted at the indicated time points from the ovine oviduct epithelial cells (n = 3). Values are given as mean ± standard deviation. (*: p < 0.05 vs control, **: p < 0.01 vs control).