Skip to main content
Figure 1 | BMC Veterinary Research

Figure 1

From: Modulation of ovine SBD-1 expression by 17beta-estradiol in ovine oviduct epithelial cells

Figure 1

E 2 up-regulates SBD-1 gene expression in ovine oviduct epithelial cells. Through QPCR, these primers are valid because of the single peak in every dissociation curve using SYBR® Green I. (A, B) Different concentrations of E2 treatment of ovine oviduct epithelial cells increased the mRNA levels of SBD-1. The z-axis or y-axis represents the signal derived from quantitative PCR on total RNA extracted from the cells. (C) The x-axis shows the time during which the cells were exposed to 10-8 M E2. Furthermore, a blank control (water substituted for cDNA) was included in each qPCR reaction mixture, and a control was set up containing water instead of E2 in every group. (E) The greatest induction was observed in the 10-8 M E2 treated group at 3.5 h. Different concentrations of E2 were added to the various groups, and total RNA extracted at the indicated time points from the ovine oviduct epithelial cells (n = 3). Values are given as mean ± standard deviation. (*: p < 0.05 vs control, **: p < 0.01 vs control).

Back to article page