Effect of hypoxia on equine mesenchymal stem cells derived from bone marrow and adipose tissue

Ranera, Beatriz; Remacha, Ana Rosa; Álvarez-Arguedas, Samuel; Romero, Antonio; Vázquez, Francisco José; Zaragoza, Pilar; Martín-Burriel, Inmaculada; Rodellar, Clementina

doi:10.1186/1746-6148-8-142

BMC Veterinary Research

Table 1 Analysis of cell cycle in BM-MSCs (n = 6) and AT-MSCs (n = 6) cultured under hypoxic or normoxic conditions

From: Effect of hypoxia on equine mesenchymal stem cells derived from bone marrow and adipose tissue

		A		B		C		D
		BM-MSC		AT-MSC		Normoxia		Hypoxia
		Normoxia	Hypoxia	Normoxia	Hypoxia	BM-MSC	AT-MSC	BM-MSC	AT-MSC
Day 1	G₀-G₁	81.88 ± 15.37	78.64 ± 10.64	84.33 ± 9.48	89.04 ± 5.98	81.88 ± 15.37	84.33 ± 9.48	78.64 ± 10.64 ^a	89.04 ± 5.98 ^b
	S	12.51 ± 10.53	14.51 ± 7.39	9.86 ± 7.17	4.99 ± 3.12	12.51 ± 10.53	9.86 ± 7.17	14.51 ± 7.39 ^a	4.99 ± 3.12 ^b
	G₂-M	5.61 ± 5.30	6.85 ± 4.70	5.80 ± 2.42	5.74 ± 3.10	5.61 ± 5.30	5.80 ± 2.42	6.85 ± 4.70	5.74 ± 3.10
Day 2	G₀-G₁	49.32 ± 4.46 ^a	62.64 ± 5.52 ^b	39.86 ± 3.26	41.33 ± 6.23	49.32 ± 4.46 ^a	39.86 ± 3.26 ^b	62.64 ± 5.5 ^a	41.33 ± 6.23 ^b
	S	33.93 ± 6.05 ^a	24.51 ± 5.29 ^b	39.07 ± 1.50	35.54 ± 7.66	33.93 ± 6.05 ^a	39.07 ± 1.50 ^b	24.51 ± 5.29 ^a	35.54 ± 7.66 ^b
	G₂-M	17.89 ± 6.99 ^a	12.85 ± 3.67 ^b	21.07 ± 3.52	23.13 ± 2,20	17.89 ± 6.99	21.07 ± 3.52	12.85 ± 3.67 ^a	23.13 ± 2,20 ^b
Day 3	G₀-G₁	56.00 ± 5.85 ^a	65.96 ± 5.83 ^b	45.37 ± 3.85	51.57 ± 5.86	56.00 ± 5.85 ^a	45.37 ± 3.85 ^b	65.96 ± 5.83 ^a	51.57 ± 5.86 ^b
	S	31.36 ± 8.24	22.53 ± 5.18	38.36 ± 4.49	31.42 ± 9.07	31.36 ± 8.24 ^a	38.36 ± 4.49 ^b	22.53 ± 5.18	31.42 ± 9.07
	G₂-M	12.94 ± 4.46	11.50 ± 3.31	14.60 ± 1.04	17.01 ± 6.58	12.94 ± 4.46	14.60 ± 1.04	11.50 ± 3.31 ^a	17.01 ± 6.58 ^b
Day 4	G₀-G₁	56.30 ± 5.65 ^a	74.13 ± 7.11 ^b	54.67 ± 2.64	52.74 ± 6.51	56.30 ± 5.65	54.67 ± 2.64	74.13 ± 7.11 ^a	52.74 ± 6.51 ^b
	S	30.59 ± 10.05 ^a	15.90 ± 6.12 ^b	30.24 ± 1.30	26.40 ± 3.42	30.59 ± 10.05	30.24 ± 1.30	15.90 ± 6.12 ^a	26.40 ± 3.42 ^b
	G₂-M	13.00 ± 5.00	9.97 ± 4.26	14.91 ± 2.34	20.19 ± 8.15	13.00 ± 5.00	14.91 ± 2.34	9.97 ± 4.26	20.19 ± 8.15
Day 5	G₀-G₁	69.77 ± 11.77	79.77 ± 7.22	65.83 ± 5.76	62.63 ± 9.25	69.77 ± 11.77	65.83 ± 5.76	79.77 ± 7.22 ^a	62.63 ± 9.25 ^b
	S	20.16 ± 13.11	11.18 ± 4.44	22.18 ± 3.15	23.85 ± 5.48	20.16 ± 13.11	22.18 ± 3.15	11.18 ± 4.44 ^a	23.85 ± 5.48 ^b
	G₂-M	10.07 ± 2.33	9.06 ± 5.04	11.99 ± 2.75	13.55 ± 4.23	10.07 ± 2.33	11.99 ± 2.75	9.06 ± 5.04	13.55 ± 4.23
Day 6	G₀-G₁	75.51 ± 15.79	83.07 ± 6.31	78.36 ± 1.89	71.49 ± 11.70	75.51 ± 15.79	78.36 ± 1.89	83.07 ± 6.31	71.49 ± 11.70
	S	16.08 ± 15.59	8.42 ± 3.02	13.16 ± 1.80	18.43 ± 9.18	16.08 ± 15.59	13.16 ± 1.80	8.42 ± 3.02	18.43 ± 9.18
	G₂-M	8.36 ± 1.67	8.35 ± 5.52	8.49 ± 1.01	10.35 ± 2.53	8.36 ± 1.67	8.49 ± 1.01	8.35 ± 5.52	10.35 ± 2.53
Day 7	G₀-G₁	81.71 ± 6.83	84.50 ± 8.33	85.78 ± 1.56	77.16 ± 12.67	81.71 ± 6.83	85.78 ± 1.56	84.50 ± 8.33	77.16 ± 12.67
Day 7	S	15.65 ± 16.21	7.78 ± 4.03	6.73 ± 1.32	14.06 ± 9.66	15.65 ± 16.21	6.73 ± 1.32	7.78 ± 4.03	14.06 ± 9.66
	G₂-M	2.64 ± 2.01	7.77 ± 4.92	7.50 ± 1.26	8.78 ± 3.20	2.64 ± 2.01	7.50 ± 1.26	7.77 ± 4.92	8.78 ± 3.20

The G₀/G₁, S and G₂/M cell cycle phases were analysed for 7 days. Cell cycle data are represented as the mean ± standard deviation. The “a” and “b” superscripted bold data are significantly different. (A) Compares normoxic and hypoxic conditions of BM-MSCs; (B) compares normoxic and hypoxic conditions of AT-MSCs; (C) compares BM-MSCs and AT-MSCs in normoxic conditions; and (D) compares BM-MSCs and AT-MSCs in hypoxic conditions.

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ISSN: 1746-6148

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