Figure 4From: Co-administration of avian influenza virus H5 plasmid DNA with chicken IL-15 and IL-18 enhanced chickens immune responsesRT-PCR analysis of total RNA extracted from transfected Vero cells. RT-PCR analysis of the transfected Vero cells showed the presence of H5 transcript in the transfected cell lysate. The products were resolved in 2% (w/v) agarose gel in TAE. M: 100 bp DNA ladder (Promega, Madison WI); Lane 1 and 2: H5 transcript (175 bp) (b) No specific band was observed when RNAs was analyzed by PCR without the RT part as in Lane 3 and 4: H5.Back to article page