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Table 5 Detection limit of virus particles with rRT-PCR assays and cRT-PCR assays a

From: Development of one-step TaqMan® real-time reverse transcription-PCR and conventional reverse transcription-PCR assays for the detection of equine rhinitis A and B viruses

ERV Strainsb

TCF Titer

Assay name

  

rRT-PCR Assays

cRT-PCR Assays

  

ERAV

ERBV

ERAV

ERBV

  

ERAV

ERBV1

ERBV2

ERAV 5'-UTR

ERAV POLY1

ERAV POLY2

ERBV 5'-UTR

ERBV OUTER 1d

ERBV OUTER 2d

ERBV INNER 1d

ERBV INNER 2d

ERBV Poly 1

ERBV Poly 2

ERAV

10−7

10−6

NAc

NA

10−6

10−5

10−5

NA

NA

NA

NA

NA

NA

NA

ERBV

10−7

NA

10−7

10−4

NA

NA

NA

10−1

10−5

10−4

10−4

10−4

10−4

10−5

  1. a Serial decimal dilutions of ERAV and ERBV were tested in a comparison study by virus isolation in cell culture, rRT-PCR and standard RT-PCR assays. Numbers shown on the table represent the serial virus dilution.
  2. b ERAV and ERBV prototype strains were obtained from NVSL.
  3. c Not applicable.
  4. d The primers used in these four assays were obtained from a nested RT-PCR developed by Black et al. (2007).