ERV Strainsb | TCF Titer | Assay name |
---|
| | rRT-PCR Assays | cRT-PCR Assays |
---|
| | ERAV | ERBV | ERAV | ERBV |
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| | ERAV | ERBV1 | ERBV2 | ERAV 5'-UTR | ERAV POLY1 | ERAV POLY2 | ERBV 5'-UTR | ERBV OUTER 1d | ERBV OUTER 2d | ERBV INNER 1d | ERBV INNER 2d | ERBV Poly 1 | ERBV Poly 2 |
---|
ERAV | 10−7 | 10−6 | NAc | NA | 10−6 | 10−5 | 10−5 | NA | NA | NA | NA | NA | NA | NA |
ERBV | 10−7 | NA | 10−7 | 10−4 | NA | NA | NA | 10−1 | 10−5 | 10−4 | 10−4 | 10−4 | 10−4 | 10−5 |
- a Serial decimal dilutions of ERAV and ERBV were tested in a comparison study by virus isolation in cell culture, rRT-PCR and standard RT-PCR assays. Numbers shown on the table represent the serial virus dilution.
- b ERAV and ERBV prototype strains were obtained from NVSL.
- c Not applicable.
- d The primers used in these four assays were obtained from a nested RT-PCR developed by Black et al. (2007).