Figure 3

ORFV-induced dispersion of the Golgi apparatus. (A) Vero cells were infected (m.o.i. 0.5) and stained with the MHC I-specific mAb W6/32 (green) and the Giantin antibody specific for Golgi (red) after 10 hpi (panels A-F) and 24 hpi (panels G-L) or 24 hpi in the presence of AraC (panels M-R). In infected cells MHC I dispersed into the cytoplasm and Golgi structures dispersed into the cytoplasm. Nuclei are stained blue by DAPI. Representative results of five independent experiments are shown. (B) The distances of the Golgi from the nucleus in non-infected and infected cells in the presence or absence of AraC were quantified using with AxioVision Rel. 4.8 software. The evaluation of 46 infected cells (ORFV), 20 infected and AraC-treated cells (ORFV/AraC), and 42 non-infected cells (ni) demonstrates significantly (T test: P < 0.0001) increased distance between Golgi and cell nucleus in infected cells. Box plots with median percentile were accomplished with GraphPad Prism 5 software.