Figure 3

Co-localization of PCV1 in monocytes (SWC3+ cells). Co-localization of PCV1 in monocytes (SWC3+ cells) of the lungs of a CCL33-inoculated foetus (Bar = 100 μm) and a 3384-inoculated foetus (Bar = 100 μm) collected at 21 days post inoculation (76 days of gestation). Methanol-fixed cryostat sections were incubated (as described in Materials and Methods) with a mAb anti-SWC3 (74.22.15) (green fluorescence). Cells containing PCV1 antigens were localized using biotin-labelled mono-specific porcine anti-PCV1 polyclonal antibodies (red fluorescence).