Table 1 Specificity testing of rt-PCR assays with isolates of the M.mycoides cluster (A), mollicute strains frequently isolated from bovine lungs (B) and bacterial strains causing CBPP-like symptoms (C)
Species | Strain | n | Host | Origin | Year | MSC_0136 rt-PCR | MSC_1046 rt-PCR | |
|---|---|---|---|---|---|---|---|---|
A | M. mycoides | PG1T | cattle | ? | ? | + | + | |
subsp. Mycoides SC | diverse | 19 | cattle | Italy | 1990-1992 | + | + | |
diverse | 3 | cattle | Spain | unknown | + | + | ||
P0 | cattle | France | 1967 | + | + | |||
N6 | cattle | Botswana | 1996 | + | + | |||
M375 | cattle | Botswana | 1995 | + | + | |||
Afade | cattle | Cameroon | 1968 | + | + | |||
PO2 | cattle | France | 1980 | + | + | |||
L2 | cattle | Italy | 1993 | + | + | |||
Fatick | cattle | Senegal | 1968 | + | + | |||
C11 | cattle | Chad | 1962 | + | + | |||
95014 | cattle | Tanzania | 1995 | + | + | |||
T1/44 | cattle | Tanzania | 1952 | + | + | |||
Gladysdale | cattle | Australia | 1953 | + | + | |||
Shawawa | cattle | Botswana | 1995 | + | + | |||
2091 | cattle | France | 1984 | + | + | |||
197 | cattle | Italy | 1992 | + | + | |||
B103 | cattle | Portugal | 1986 | + | + | |||
6305 | cattle | Portugal | 1993 | + | + | |||
6526 | cattle | Portugal | 1993 | + | + | |||
Astercous | cattle | Spain | 1987 | + | + | |||
Madrid | cattle | Spain | 1993 | + | + | |||
Tan8 | cattle | Tanzania | 1998 | + | + | |||
Matapi | cattle | Namibia | 2004 | + | + | |||
Mandigwan | cattle | Namibia | 2001 | + | + | |||
V5 | cattle | Australia | 1936 | + | + | |||
IS31 | cattle | Tanzania | 1998 | + | + | |||
2022 | cattle | France | 1984 | + | + | |||
B237 | cattle | Kenya | 1987 | + | + | |||
M. mycoides | PG3, NCTC 10137 T | goat | Turkey | 1950 | - | - | ||
subsp. capri | Y-Goat | goat | Australia | 1956 | - | - | ||
7302 | goat | Portugal | < 1994 | - | - | |||
Kombolcha | goat | Ethiopia | 1975 | - | - | |||
7730 | goat | France | < 1997 | - | - | |||
9096-C9415 | goat | Nigeria | unknown | - | - | |||
diverse | 6 | goat | Gran Canaria | 1993 | - | - | ||
My-I | goat | Croatia | 1986 | - | - | |||
D2482 | goat | Switzerland | 1991 | - | - | |||
95010 | goat | France | 1995 | - | - | |||
6443-90 | goat | France | 1990 | - | - | |||
My-325 | goat | Croatia | 1986 | - | - | |||
C260/4 | goat | Gran Canaria | 1993 | - | - | |||
Wk354 | goat | Switzerland | 1980 | - | - | |||
L | goat | France | 1975 | - | - | |||
Wi18079 | goat | Germany | 2009 | - | - | |||
diverse | 3 | Barbary sheep | Germany | 1994 | - | - | ||
M-5 | goat | Croatia | 1988 | - | - | |||
M-18 | goat | Croatia | 1988 | - | - | |||
M-29 | goat | Croatia | 1988 | - | - | |||
M. capricolum | Cal. kid, NCTC 10154 T | goat | USA | 1954 | - | - | ||
subsp. capricolum | C47 | sheep | Germany | < 1992 | - | - | ||
4146 | goat | France | 1980 | - | - | |||
7714 | goat | France | 1967 | - | - | |||
609-79 | goat | France | 1979 | - | - | |||
8086-1 | goat | France | 1980 | - | - | |||
M. capricolum | F38, NCTC 10192 T | goat | Kenya | 1976 | - | - | ||
subsp. capripneumoniae | 87F05 | goat | Turkey | 2005 | - | - | ||
diverse | 2 | gazelle | UAE | 2004 | - | - | ||
3535 | mouflon | Qatar | 2005 | - | - | |||
GL97P | goat | Tunisia | 1980 | - | - | |||
M. leachii | PG50, NCTC 10133 T | cattle | Australia | 1963 | - | - | ||
D318b | cattle | Germany | unknown | - | - | |||
D424 | cattle | Germany | unknown | - | - | |||
Calf1 | cattle | Nigeria | unknown | - | - | |||
M.nov.spec. | 8756-13 | mountain goat | USA | 1987 | - | - | ||
G1650 | capricorn | Germany | 1994 | - | - | |||
G5847 | capricorn | Germany | 1993 | - | - | |||
B | M. putrefaciens | KS1, NCTC 10155 T | goat | USA | 1955 | - | - | |
7578.94 | goat | France | 1994 | - | - | |||
Tours2 | goat | France | 1972 | - | - | |||
M. bovis | PG45, NCTC 10131 T | cattle | USA | 1962 | - | - | ||
363/88 | cattle | Germany | 1988 | - | - | |||
334/96 | cattle | Germany | 1996 | - | - | |||
54/10 | cattle | Germany | 2010 | - | - | |||
M. bovirhinis | PG43, NCTC 10118 T | cattle | UK | 1967 | - | - | ||
320/93 | cattle | Germany | 1993 | - | - | |||
19/94 | cattle | Germany | 1994 | - | - | |||
365/09 | cattle | Germany | 2009 | - | - | |||
M. bovigenitalium | PG11, NCTC 10122 T | cattle | UK | 1947 | - | - | ||
338/87 | cattle | Germany | 1987 | - | - | |||
18/92 | cattle | Germany | 1992 | - | - | |||
466/94 | cattle | Germany | 1994 | - | - | |||
M. arginini | G230, NCTC 10129 T | mouse | USA | 1968 | - | - | ||
162/81 | cattle | Germany | 1981 | - | - | |||
208/94 | Barbary sheep | Germany | 1994 | - | - | |||
23/05 | sheep | Germany | 2005 | - | - | |||
M. californicum | St-6, NCTC 10189 T | cattle | USA | 1981 | - | - | ||
456/83 | cattle | Germany | 1983 | - | - | |||
111/84 | cattle | Germany | 1984 | - | - | |||
M. canadense | 275C, NCTC 10152 T | cattle | Canada | 1974 | - | - | ||
380/94 | cattle | Germany | 1994 | - | - | |||
M. verecundum | 107, NCTC 10145 T | cattle | UK | 1974 | - | - | ||
49/94 | cattle | Germany | 1994 | - | - | |||
M. alkalescense | PG51, NCTC 10135 T | cattle | Australia | 1961 | - | - | ||
26/93 | cattle | Germany | 1993 | - | - | |||
M. canis | PG14, NCTC 10146 T | dog | UK | 1951 | - | - | ||
306/93 | dog | Germany | 1993 | - | - | |||
A. laidlawii | PG8, NCTC 10116 T | unknown | UK | 1967 | - | - | ||
54/99 | cattle | UK | 1999 | - | - | |||
A. axanthum | S743, NCTC 10138 T | tissue culture | UK | 1970 | - | - | ||
824/80 | cattle | Germany | 1980 | - | - | |||
Ureaplasma diversum | A417, NCTC 10182 T | cattle | UK | 1972 | - | - | ||
C | Pasteurella multocida | ATCC 43137 T | pig | - | - | |||
Mannheimia haemolytica | ATCC 33396 T | unknown | - | - |