A novel integrase-containing element may interact with Laem-Singh virus (LSNV) to cause slow growth in giant tiger shrimp

Table 1 RT-PCR primers used to amplify the portions of the ICE sequence

Primer names	Melting temperature (Tm, °C)	Expected product (bp)
Probe 1-Fr	57.56	397
Probe1-Rv	57.80
Probe 2-Fr	60.40	642
Probe 2-Rv	58.40
Probe 3-Fr	58.55	399
Probe 3-Rv	58.70
LSNV-Fr LSNV-Rv	60 61	600
Nested primers
Probe 3-NFr	60	204
Probe 3-NRv	60
LSNV-NFr	64	195
LSNV-NRv	66

ISSN: 1746-6148