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Table 3 List of primers used for RT-PCR. For each fragment, forward and reverse primers were used

From: The role of African buffalos (syncerus caffer) in the maintenance of foot-and-mouth disease in Uganda

Sample ID

Forward Primers (5' to 3')

Reverse primers (5' to 3')

BUF 10

CAGTACTCCGGCAGCCTG

GGTGTTGTAATTGCACTCTCC

 

CAGTGGTGTTCTCGCACAAC

GCCATDGGMGGGATGAACCC

BUF 6

GACCGTATTCTCACCACGAG

AAGTTGGACCTGACGTCGG

BUF 17

CAAAXAGGGAATTTTXCCCGTXGC

GACGACXGGXTTGTCGCC

 

CTGGTXGGCGCAATCCTXCGT

CGGTTRAAGTCGGGWCCGTG

  1. The sequences obtained from samples BUF 10, BUF 6 and BUF 17 were subsequently named SAT 2/UGA/1/07, SAT 2/UGA/2/07 and SAT 1/UGA/1/07, respectively. These samples were all collected on the same day (17/1/07) in Queen Elizabeth National Park, but BUF 17 was from a different herd.
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BMC Veterinary Research

ISSN: 1746-6148

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