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Figure 3 | BMC Veterinary Research

Figure 3

From: Antibody response to a sterile filtered PPD tuberculin in M. bovis infected and M. bovis sensitized cattle

Figure 3

2-DE analysis of SF-PPD and HK-PPD. 10 μg of either SF-PPD (a) or HK-PPD (b) were loaded onto 17 cm pre-cast acrylamide strips with an immobilized pH range of 3-10 (ReadyStrip™; Bio-Rad). Following isoelectric focussing, the acrylamide strips were loaded into vertical 12% polyacrylamide gels with molecular weight standards (Precision Plus, Bio-Rad). The gels were stained with a non-fixing silver stain.

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