PCR condition | |||||||
---|---|---|---|---|---|---|---|
Target gene | Primers: Forward and reverse | Amplicon bp | Acc. Number | AT | Nm | R2 | Slope |
SCD | F-5'cccagctgtcagagaaaagg-3' R-5'gatgaagcacaacagcagga-3' | 115 | AJ001048 | 59 | 900 900 | 0.996 | -3.35 |
SREBP1 | F-5' ctgctatgcaggcagcac-3' R-5' ggttgatgggcagcttgt-3' | 99 | GU206528 | 59 | 900 900 | 0.980 | -3.32 |
PPARγ | F-5'cttgctgtggggatgtctc-3' R-5'ggtcagcagactctgggttc-3' | 121 | AY137204 | 60 | 900 900 | 0.982 | -3.35 |
PPARα | F-5'tgccaagatctgaaaaagca-3' R-5' cctcttggccagagacttga-3' | 99 | FJ200440.1 | 59 | 300 300 | 0.98 | -0.38 |
ACTB | F-5'ggacctgacggactacctcatg-3' R-5'ggccatctcctgctcgaagt-3' | 136 | U39357 | 60 | 300 300 | 0.989 | -3.32 |
SDHA | F-5'catccactacatgacggagca-3' R-5'atcttgccatcttcagttctgcta-3' | 90 | AY970969 | 60 | 300 300 | 0.990 | -3.36 |
G6PDH | F-5'tgacctatggcaaccgatacaa-3' R-5' ccgcaaaagacatccaggat-3' | 76 | DQ377364 | 60 | 900 900 | 0.994 | -3.33 |
UBC | F-5 cgtcttaggggtggctgtta-3' R-5'aaattggggtaaatggctaga-3' | 90 | NM_001009202 | 59 | 600 600 | 0.991 | -3.51 |