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Table 3 Genes and real-time amplification products.

From: Effect of the feeding system on the fatty acid composition, expression of the Δ9-desaturase, Peroxisome Proliferator-Activated Receptor Alpha, Gamma, and Sterol Regulatory Element Binding Protein 1 genes in the semitendinous muscle of light lambs of the Rasa Aragonesa breed

    

PCR condition

Target gene

Primers: Forward and reverse

Amplicon bp

Acc. Number

AT

Nm

R2

Slope

SCD

F-5'cccagctgtcagagaaaagg-3'

R-5'gatgaagcacaacagcagga-3'

115

AJ001048

59

900

900

0.996

-3.35

SREBP1

F-5' ctgctatgcaggcagcac-3'

R-5' ggttgatgggcagcttgt-3'

99

GU206528

59

900

900

0.980

-3.32

PPARγ

F-5'cttgctgtggggatgtctc-3'

R-5'ggtcagcagactctgggttc-3'

121

AY137204

60

900

900

0.982

-3.35

PPARα

F-5'tgccaagatctgaaaaagca-3'

R-5' cctcttggccagagacttga-3'

99

FJ200440.1

59

300

300

0.98

-0.38

ACTB

F-5'ggacctgacggactacctcatg-3'

R-5'ggccatctcctgctcgaagt-3'

136

U39357

60

300

300

0.989

-3.32

SDHA

F-5'catccactacatgacggagca-3'

R-5'atcttgccatcttcagttctgcta-3'

90

AY970969

60

300

300

0.990

-3.36

G6PDH

F-5'tgacctatggcaaccgatacaa-3'

R-5' ccgcaaaagacatccaggat-3'

76

DQ377364

60

900

900

0.994

-3.33

UBC

F-5 cgtcttaggggtggctgtta-3'

R-5'aaattggggtaaatggctaga-3'

90

NM_001009202

59

600

600

0.991

-3.51

  1. Genes, primers (F: forward and R: reverse), length of the amplicon and GenBank accession (acc.) numbers for the ovine sequences. Real-time RT-PCR conditions: annealing temperature (AT), primer concentrations (Nm), correlation coefficient (R2) and slope of the standard curve.