From: Differential gene expression in brain tissues of aggressive and non-aggressive dogs
Gene symbol | Gene name | Accession no. | Gene function* | Primer | Amplicon size (bp) | PCR efficiency |
---|---|---|---|---|---|---|
Target genes | ||||||
SCD | stearoyl-CoA desaturase | XM_543968.2 | synthesis of unsaturated fatty acids | Forward: CTCTACGGATATCGCCCTTACG | 106 | 1.93 |
Reverse: GAAAGGAGTGGTGGTAGTTGTGG | ||||||
TMEM47 | transmembrane protein 47 | NM_001003045.1 | cellular component - plasma membrane | Forward: TGGTGGGTTTGATTTCTATCTGC | 123 | 1.94 |
Reverse: TGAACTTGATTGGGTAAAGGACC | ||||||
ATP5A1 | ATP synthase | XM_861390.1 | catalyzes ATP synthesis | Forward: TCCATGACGGAACTGATGAA | 165 | 1.94 |
Reverse: ACTGAAGTGGAGCAGCATCA | ||||||
CALM 2 | calmodulin2 | XM_859583.1 | calcium ion binding | Forward: GATGAAATGATCAGGGAAGCA | 178 | 1.93 |
Reverse: AGCAGGGGGAAACCTTTTAC | ||||||
UBE2V2 | TMEM189-UBE2V1 | XM_544068.2 | small conjugating protein ligase activity | Forward: AGGAGGAAAAATGCAGCACA | 144 | 1.92 |
Reverse: AACACACATGCTTCCACAGC | ||||||
ZNF 227 | zinc finger protein 227 | XM_850112.1 | nucleic acid binding, zinc ion binding | Forward: CATCTTCCCTTCAAGCCAGA | 119 | 2.1 |
Reverse: TTTCGAAGTGTCTCCATCTCA | ||||||
LOC488837 | similar to T05C3.2 | XM_545955.2 | apoptosis, protein-protein interactions** | Forward: CCGGAAAATTGCTAACATTGA | 115 | 1.91 |
Reverse: CAGCTTCTCTTGGGCTTCTG | ||||||
RIMBP2 | RIMS binding protein 2 | XM_543356.2 | interaction in synaptic-vesicle release and presynaptic voltage-gated calcium channels*** | Forward: CAGCTTCTGAGACAAGGCTTCC | 124 | 1.97 |
Reverse: TCGGGTCGTAATCATACAGGG | ||||||
GLT1 | solute carrier family 1 member2 | NM_001003138 | synaptic clearance of glutamate | Forward: GGTCATCCTGGTCTTGGCTA | 259 | 1.74 |
Reverse: CTCCGTCACAGTCTCGTTCA | ||||||
Endogenous control genes | ||||||
LOC482993 (RPL32) | similar to 60S ribosomal protein L32 | XM_540107 | protein binding, structural constituent of ribosome | Forward: ATGCCCAACATTGGTTACGG Reverse: CTCTTTCCACGATGGCTTTG | 181 | 1.84 |
HNRNPH1 | heterogeneous nuclear ribonucleoprotein H1 | XM_852029.1 | RNA binding | Forward: CTCACTATGATCCACCACG Reverse: TAGCCTCCATAACCTCCAC | 151 | 1.81 |