Stage | Primer orientation | Sequence (5' - 3') |
---|---|---|
1st round nested PCR | SqP1 - forward SqP2 - reverse | GCGGCCGCGCTGACCGCCATCG CCAGCACGACTTCTTCCTGGAG |
2nd round nested PCR | SqP3 - forward SqP4 - reverse | CGCTCGCGTGTCCTACAGCCTG GCAGTCCGCAGCGCGCGCAGAT |
Real-time PCR primers | SP7 - forward SP8 - reverse | GGGCGATCGTGCCGCTCAG CGCCTCGAGGTCCGGCTC |