Skip to main content

Table 1 Comparison of nested and real-time PCR results from 39 grey skin samples

From: Detection of squirrel poxvirus by nested and real-time PCR from red (Sciurus vulgaris) and grey (Sciurus carolinensis) squirrels

 

Nested PCR results

 

Positive

Negative

Real-time PCR results

Positivea

13

3

 

Negativeb

4

15

 

Equivocalc

1

3

  1. a 3/3 or 2/3 of the triplicates positive Ct and appropriate melt curve.
  2. b 0/3 positive Ct and no melt curve.
  3. c 1/3 positive Ct and melt curve.