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Table 4 Primers used for amplification and sequencing of PRRSV (Lena) and PRRSV (US5)

From: Pathogenesis and antigenic characterization of a new East European subtype 3 porcine reproductive and respiratory syndrome virus isolate

Target

Sequence

Location*

Direction

Target

Sequence

Location*

Direction

Lena

   

US5

   

ORF2a

5'-gtsacaccktatgattacg-3'

11471-11489

Forward

    
 

5'-tcatrccctattytgcacca-3'

12642-12623

Reverse

    

ORF4

5'-cggcccaittccatccigag-3'

12756-12775

Forward

ORF4

5'-cggcccaittccatccigag-3'

12756-12775

Forward

 

5'-cattcagctcgcataicgtcaag-3'

13653-13631

Reverse

 

5'-cattcagctcgcataicgtcaag-3'

13653-13631

Reverse

ORF5

5'-tgcticatttcitgacacc-3'

13418-13436

Forward

ORF5

5'-tgcticatttcitgacacc-3'

13418-13436

Forward

 

5'-accttaagigcitatatc-3'

14190-14173

Reverse

 

5'-accttaagigcitatatc-3'

14190-14173

Reverse

ORF6

5'-atgggaggcctagacgatttt-3'

14087-14107

Forward

ORF6

5'-taccaacttcattgtggac-3'

13922-13940

Forward

 

5'-ccggccatacttgacgaggt-3'

14605-14586

Reverse

 

5'-acccagcaactggcacag-3'

14690-14673

Reverse

ORF7

5'-tggcccctgcccaicacg-3'

14412-14429

Forward

ORF7

5'-tggcccctgcccaicacg-3'

14412-14429

Forward

 

5'-tcgccctaattgaataggtga-3'

15050-15030

Reverse

 

5'-tcgccctaattgaataggtga-3'

15050-15030

Reverse

  1. *Locations are derived from Lelystad virus