Table 2 Identification of proteins from equine plasma in reducing 1-D SDS-PAGE.
From: Biomarkers for ragwort poisoning in horses: identification of protein targets
Band No. | Protein Identification | Accession No. | Mr (kDa) | pI | MOWSE Scorea (p≤ 0.05) | No. of Peptides Matched | % Coverageb | Species |
|---|---|---|---|---|---|---|---|---|
R1 | Serum albumin | CAA52194 | 70.5 | 5.95 | 437 (44) | 48 | 30 | Equus caballus |
| Â | Immunoglobulin gamma 7 heavy chain | AAS18414 | 36.3 | 7.70 | 132 (44) | 8 | - | Equus caballus |
| Â | Immunoglobulin gamma 4 heavy chain | AAS18415 | 36.2 | 7.71 | 127 (44) | 10 | - | Equus caballus |
|  | Fibrinogen A α chain (fragment) | AAC67561 | 49.6 | 6.45 | 49 (45) | 3 | 7 | Equus caballus |
R2 | Serum albumin | CAA52194 | 70.6 | 5.95 | 639 (44) | 39 | 31 | Equus caballus |
|  | Fibrinogen A α chain (fragment) | AAC67561 | 49.6 | 6.45 | 230 (44) | 14 | 19 | Equus caballus |
| Â | Immunoglobulin G heavy chain (fragment) | AAG01011 | 47.6 | 5.7 | 141 (44) | 8 | 14 | Equus caballus |
| Â | Immunoglobulin G light chain (fragment) | AAG01010 | 24.5 | 6.4 | 141 (44) | 4 | 9 | Equus caballus |
| Â | Apolipoprotein A-1 | P02648 | 30.2 | 5.2 | 94 (44) | 6 | 7 | Canis familiaris |
R3 | Transferrin | AAA63684 | 80.3 | 6.83 | 685 (44) | 47 | 32 | Equus caballus |
|  | Fibrinogen A α chain | AAC67561 | 49.6 | 6.45 | 195 (44) | 15 | 26 | Equus caballus |
| Â | Serum albumin | CAA52194 | 70.6 | 5.95 | 187 (44) | 16 | 14 | Equus caballus |
| Â | Immunoglobulin mu | AAU09792 | 50.1 | 5.52 | 127 (44) | 10 | 16 | Equus caballus |
R4 | Serum albumin | AAV28861 | 70.5 | 5.89 | 249 (45) | 18 | 16 | Equus caballus |
|  | Fibrinogen A α chain | AAC67561 | 49.6 | 6.45 | 50 (45) | 3 | 5 | Equus caballus |
R5 | Immunoglobulin gamma 7 heavy chain | AAS18414 | 36.3 | 7.7 | 305 (44) | 25 | Â | Equus caballus |
| Â | Immunoglobulin gamma 4 heavy chain constant region (fragment) | CAC44763 | 37.0 | 8.04 | 280 (44) | 24 | 32 | Equus caballus |
| Â | Immunoglobulin gamma 5 heavy chain constant region (fragment) | CAC86340 | 36.4 | 5.95 | 256 (44) | 24 | 33 | Equus caballus |
| Â | Immunoglobulin G heavy chain (fragment) | AAG01011 | 47.6 | 5.7 | 256 (44) | 22 | 21 | Equus caballus |
| Â | Immunoglobulin gamma 4 heavy chain | AAS18415 | 36.2 | 7.71 | 247 (44) | 24 | - | Equus caballus |
| Â | Immunoglobulin gamma 1 heavy chain constant region (fragment) | CAC44624 | 38.0 | 7.68 | 224 (44) | 11 | 19 | Equus caballus |
| Â | Immunoglobulin gamma 6 heavy chain constant region (fragment) | CAC86341 | 36.5 | 8.03 | 212 (44) | 13 | 24 | Equus caballus |
| Â | Serum albumin | CAA52194 | 70.6 | 5.95 | 92 (44) | 6 | 4 | Equus caballus |
| Â | Haptoglobin | CAA25267 | 39.0 | 6.13 | 75 (45) | 5 | - | Homo sapiens |
R6 | Immunoglobulin gamma 7 heavy chain | AAS18414 | 36.3 | 7.7 | 305 (44) | 25 | Â | Equus caballus |
| Â | Immunoglobulin gamma 4 heavy chain constant region (fragment) | CAC44763 | 37.0 | 7.28 | 280 (44) | 24 | 32 | Equus caballus |
| Â | Immunoglobulin gamma 4 heavy chain | AAS18415 | 36.2 | 7.71 | 247 (44) | 24 | - | Â |
| Â | Immunoglobulin gamma 1 heavy chain constant region (fragment) | CAC44624 | 38.0 | 7.68 | 224 (44) | 11 | 19 | Equus caballus |
| Â | Immunoglobulin gamma 6 heavy chain constant region (fragment) | CAC86341 | 36.5 | 8.03 | 212 (44) | 13 | 24 | Equus caballus |
R7 | Immunoglobulin gamma 4 heavy chain | AAS18415 | 36.2 | 7.71 | 93 (44) | 3 | 10 | Equus caballus |
| Â | Immunoglobulin gamma 4 heavy chain constant region (fragment) | CAC44763 | 37.0 | 7.28 | 93 (44) | 3 | 10 | Equus caballus |
R8 | Apolipoprotein A-1 | P02648 | 30.1 | 5.2 | 608 (44) | 36 | 27 | Canis familiaris |
| Â | Lambda immunoglobulin (fragment) | AAA50978 | 17.6 | 8.35 | 295 (44) | 17 | 23 | Equus caballus |
| Â | Lambda immunoglobulin (fragment) | AAA50971 | 20.7 | 8.22 | 192 (44) | 7 | 24 | Equus caballus |