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Table 1 Identification of proteins from equine plasma in non-reducing 1-D SDS-PAGE. The plasma proteins were identified using LC-MS/MS.

From: Biomarkers for ragwort poisoning in horses: identification of protein targets

Band No.

Protein Identification

Accession No.

Mr (kDa)

pI

MOWSE Score a (p ≤ 0.05)

No. of Peptides Matched

% Coverageb

Species

NR1

Serum albumin

CAA52194

70.6

5.95

403 (44)

41

34

Equus caballus

 

Fibrinogen A α chain (fragment)

AAC67561

49.6

6.45

138 (44)

11

7

Equus caballus

 

Immunoglobulin gamma 4 heavy chain constant region (fragment)

CAC44763

37.0

8.04

147 (44)

15

20

Equus caballus

 

Immunoglobulin gamma 7 heavy chain

AAS18414

36.3

7.70

109 (44)

13

-

Equus caballus

 

Immunoglobulin gamma 4 heavy chain

AAS18415

36.2

7.71

106 (44)

15

-

Equus caballus

 

Transferrin

AAA63684

80.3

6.83

102 (44)

5

6

Equus caballus

NR2

Serum albumin

CAA52194

70.6

5.95

477 (45)

34

41

Equus caballus

 

Immunoglobulin mu

AAU09792

50.1

5.52

372 (45)

24

44

Equus caballus

 

Lambda immunoglobulin (fragment)

AAA50978

17.7

8.35

165 (45)

9

31

Equus caballus

 

Fibronectin

AAC48614

58.1

5.91

124 (45)

3

7

Equus caballus

NR3

Fibrinogen A α chain (fragment)

AAC67561

49.6

6.45

754 (44)

42

45

Equus caballus

 

Serum albumin

CAA52194

68.6

5.95

265 (44)

22

24

Equus caballus

 

Fibrinogen γ B chain

CAA33562

50.8

5.54

121 (44)

6

6

Bos taurus

NR4

Lamba Immunoglobulin (fragment)

AAA50978

17.4

8.35

274 (44)

15

32

Equus caballus

 

Immunoglobulin gamma 5 heavy chain constant region (fragment)

CAC86340

35.9

5.95

272 (44)

20

28

Equus caballus

 

Immunoglobulin gamma 1 heavy chain constant region (fragment)

CAC44624

37.4

7.68

270 (44)

7

17

Equus caballus

 

Serum albumin

CAA52194

68.6

5.95

265 (44)

22

24

Equus caballus

NR5

Immunoglobulin gamma 1 heavy chain constant region (fragment)

CAC44624

37.4

7.68

554 (44)

26

34

Equus caballus

 

Immunoglobulin G heavy chain (fragment)

AAG01011

47.6

5.70

411 (44)

30

27

Equus caballus

NR6

Serum albumin

CAA52194

70.6

5.95

717 (44)

59

48

Equus caballus

 

Immunoglobulin gamma 4 heavy chain

AAS18415

36.2

7.71

463 (44)

59

-

Equus caballus

 

Immunoglobulin gamma 7 heavy chain

AAS18414

36.3

7.70

454 (44)

56

-

Equus caballus

 

Immunoglobulin gamma 4 heavy chain constant region (fragment)

CAC44763

37.0

8.04

374 (44)

46

37

Equus caballus

 

Lambda immunoglobulin (fragment)

AAA50978

7.7

8.35

337(44)

52

-

Equus caballus

NR7

Gelsolin

Q28372

80.9

5.58

354(44)

15

20

Equus caballus

NR8

Transferrin

AAA63684

80.3

6.83

421 (44)

31

27

Equus caballus

 

Carboxylesterase 1

AAH21150

63.0

5.64

114 (44)

6

6

Mus musculus

NR9

Transferrin

AAA63684

80.3

6.83

665 (44)

51

32

Equus caballus

 

α-1-antitrypsin

AAC83412

47.1

5.23

150 (44)

7

8

Equus caballus

 

Serum albumin

AAV28861

70.5

5.89

95 (44)

8

12

Equus asinus

  1. Proteins were identified on the basis of their MOWSE score using the MSDB database. Each band number refers to proteins identified in Figure 2A. a = MOWSE baseline significance score in brackets; b = for those with no percentage coverage value the database did not give this information.