Figure 3

The effect of pyrrole concentration on fibrinogen standard. Varying concentrations of DHM were incubated with a bovine fibrinogen standard, which was then analysed using reducing 1-D SDS-PAGE for 120 mins at room temperature. The proteins were separated on 7.5% (w/v) gels and visualised with Coomassie brilliant blue. Higher concentrations of DHM caused the modification of fibrinogen, whereas lower concentrations showed no effect. The α- β- and γ-chains of bovine fibrinogen are labelled. Key: - = control reaction mixture complete apart from DHM; + = reaction mixture containing DHM.