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Table 1 Akabane virus strains isolated during an outbreak of bovine encephalomyelitis and used in this study

From: Bovine epizootic encephalomyelitis caused by Akabane virus in southern Japan

    Source    
Strain Passage levela Geographical origin     
    Specimen for isolation Date collected Symptom Profile of cattle
KM-1/Br/06 Sm1, HL4 Kumamoto Brain 3 October, 2006 Astasia, Tachypnea Holstein, 4 days old
KM-2/Br/06 HL4 Kumamoto Brain 28 September, 2006 Astasia, Tachypnea, Tremor, Opisthotonus Holstein, female, 4 months old
KM-3/Ce/06 HL3 Kumamoto Cerebellum 19 October, 2006 Astasia Holstein, 5 months old
KM-6/Br/06 HL3 Kumamoto Brain stem 26 October, 2006 Astasia Holstein, female, 19 months old
KM-7/Pt/06 HL4 Kumamoto Spinal cord (Pars thoracia) 26 October, 2006 Astasia Holstein, female, 19 months old
KM-8/Br/06 HL4 Kumamoto Brain stem 18 October, 2006 Astasia Holstein, female, 16 months old
KM-9/B/06 HL3 Kumamoto Blood 30 October, 2006 No symptoms were observed when a blood sample was taken. Astasia appeared on 13 November, 2006. Holstein, 3 months old
KM-10/B/06 HL3 Kumamoto Blood September, 2006 No symptoms  
KM-11/B/06 HL2 Kumamoto Blood September, 2006 No symptoms  
KS-1/P/06 HL4 Kagoshima Plasma 12 October, 2006 No symptoms Japanese Black, castrated male, 10 months old
KS-2/Mo/06 HL5 Kagoshima Brain stem (Medulla oblongata) 18 October, 2006 Dysstasia, Ataxia Japanese Black, castrated male, 6 months old
KSB-3/P/06 BHK4 Kagoshima Plasma 27 October, 2006 No symptoms Japanese Black, female, 24 months old
  1. a Letters indicate cell lines and numbers indicate number of passages. HL, HmLu-1 cells; BHK, BHK-21 cells; Sm, suckling-mouse brain (only for isolation of KM-1/Br/06).