From: Identification of major cell types in paraffin sections of bovine tissues
Marker | clone/type | Immunogen | Source | Ref. | Staining | Dilution (method) | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
 |  |  |  |  | PFA | EtOH |  | |||||
 |  |  |  |  | N | P | H3 | H6 | H9 | N | P |  |
EPITHELIUM, ENDOTHELIUM | ||||||||||||
collagenType IV | M3F7 | human collagen | DSHB | [10] | - | - | - | - | - | - | - | 1:200T |
cytokeratin, HMW | 34betaE12 | human epid. keratin | Dako | [5] | Â | Â | Â | ++ | - | ++ | Â | 1:1000T |
cytokeratin, pan | rabbit polycl. | bovine epid. keratin | Zymed | n.a. | + | + | Â | + | Â | + | Â | 1:1000T |
eNos/NOSType III | rabbit polycl. | peptide (human) | BD | [11] | Â | Â | + | - | + | + | Â | 1:1000T |
keratin, pan | AE1 + AE3 | human epid. keratin | N/L | [2,3] | - | ++ | - | ++ | ++ | ++ | Â | 1:100A, 1:1000T |
keratin, pan | Lu-5 | lung cancer cell line | N/L | [4] | Â | ++ | Â | ++ | - | ++ | Â | 1:100A, 1:1000T |
lectin GSL I-B4 | G. simplicifolia | n.a. | Vector | [8,9] | ++ | ++ | Â | ++ | Â | ++ | Â | 1:4000T |
lectin ML-I | Viscum album | n.a. | W/H | [7] | ++ | ++ | ++ | ++ | ++ | Â | Â | 1:2000T |
von Willebrand | rabbit polycl. | human vWF | Dako | [6] | + | Â | Â | Â | Â | Â | ++ | 1:400A, 1:800T |
CONNECTIVE TISSUE | ||||||||||||
procollagenType I | SP1.D8 | ovine aminopropept. | DSHB | [14] | + | + | + | + | ++ | - | - | 1:2000T |
vimentin | V9 | porcine vimentin | Dako | [12,13] | ++ | Â | Â | ++ | Â | Â | ++ | 1:100A, 1:500T |
MUSCLE | ||||||||||||
actin, muscle | HHF35 | n.a. | Enzo | [17] | + | Â | ++ | + | ++ | ++ | ++ | 1:100A |
actin, smooth muscle α | 1A4 | peptide | Dako | [18] |  |  |  | ++ | + |  |  | 1:500T |
desmin | D33 | human desmin | Dako | [15,16] | + | Â | Â | ++ | Â | ++ | Â | 1:400A, 1:2000T |
NEURONAL TISSUE | ||||||||||||
CNPase | 11-5B | human brain CNPase | Ch | n.a. | Â | ++ | - | ++ | - | - | Â | 1:400A |
GFAP | rabbit polycl. | human GFAP | Zymed | n.a. | ++ | ++ | + | ++ | ++ | ++ | Â | 1:200A |
NeuN | A60 | mouse neuronal nuclei | Ch | [19,22] | + | + | - | + | ++ | ++ | + | 1:4000T |
NF 160/200 kD | RMdO-20 | rat neurofilaments | Zymed | [22] | ++ | Â | - | - | ++ | ++ | Â | 1:400A |
NF, pan | SMI311 (cockt) | n.a. | SM | [21] | ++ | Â | + | ++ | ++ | + | Â | 1:1000A, 1:2000T |
O4 | 81 | bovine brain | Roche | [23] | Â | Â | - | - | - | Â | - | 1:2A |
S100 | rabbit polycl. | bovine S100 | Dako | [24] | Â | Â | Â | ++ | Â | Â | Â | 1:800A |
tubulin βIII | rabbit polycl. | peptide (rat) | b/C | n.a. |  |  | - | ++ | - | + |  | 1:6000A,1:10000T |
tubulin βIII | TU-20 | n.a. | Ch | [20] | ++ |  | ++ | ++ | ++ | ++ |  | 1:400A, 1:3000T |
LEUKOCYTES | ||||||||||||
CD11a/18 | MUC76A | sheep, pig leukocytes | VMRD | [28] | - | Â | - | - | - | Â | Â | 1:40A |
CD11a/18 | BAT75A | ruminant leukocytes | VMRD | [27] | - | Â | ++ | - | - | + | Â | 1:40A, 1:200T |
CD11b | MM10A | n.a. | VMRD | [32] | Â | Â | ++ | Â | Â | ++ | Â | 1:500T |
CD14 | MM61A | bovine mononucl. cells | VMRD | [25] | + | Â | Â | Â | Â | + | Â | 1:200 |
CD34 | rabbit polycl. | peptide (human) | SC | n.a. | Â | Â | + | - | - | Â | Â | 1:200A, 1:200T |
CD3ε | rabbit polycl. | peptide (human) | Dako | [29] | - |  |  | ++ |  | + | ++ | 1:100A, 1:1000T |
CD45 | CACTB51A | bovine act. lymphocytes | VMRD | [25] | Â | + | + | + | + | + | ++ | 1:800T |
CD45 | CC1 | n.a. | Serotec | [26] | Â | Â | Â | + | + | Â | Â | 1:100A |
CD68 | EBM11 | human macrophages | Dako | [33] | - | + | Â | - | Â | Â | ++ | 1:80A |
CD79αcy | HM57 | peptide (human) | Dako | [30] |  |  | - | + | ++ |  |  | 1:500T |
IgA | rabbit polycl. | bovine IgA | BL | n.a. | Â | ++ | Â | Â | ++ | Â | ++ | 1:100A |
IgM | rabbit polycl. | bovine IgM | BL | n.a. | + | Â | Â | Â | ++ | Â | Â | 1:1500A |
IgM | BIg73A | bovine Ig | VMRD | [31] | Â | Â | - | + | ++ | Â | Â | 1:5000A |
lysozyme | rabbit polycl. | human lysozyme | Bm | [34] | Â | + | - | - | + | + | + | 1:500T |
CELL STATUS | ||||||||||||
cleaved caspase 3 (Asp175) | rabbit polycl. | peptide (human) | CST | [37] | Â | Â | Â | ++ | + | Â | Â | 1:75A |
Ki67 antigen | MIB-1 | peptide (human) | Dako | [35,36] | Â | Â | Â | - | ++ | Â | Â | 1:4000T |