Table 1 Primers and cycle conditions used in this study
From: Protection of rabbits against enteropathogenic Escherichia coli(EPEC) using an intimin null mutant
Primer | Sequence | Number of cycles (Cycle conditions)1 |
|---|---|---|
EAE FOR | 5'AAAAAAGGATCCAAAAATTACGCCGGAAGAT | 30 (15" 94Ā°C; 30" 55.4Ā°C; and 2' 68Ā°C) |
EAE REV | 5'AAAAAATCTAGAAAAAGGTAAACAGGTCTC | Ā |
248 FOR | 5'CCACTCAAAAAACTGTCTGTTG | 25 (15" 94Ā°C; 30" 58.1Ā°C; and 1' 72Ā°C) |
248 REV | 5'TCCAGTGAACTACCGTCAAAG | Ā |
ESPB FOR | 5'GGATCCGAATACTATTGATTATACTAAT | 30 (1' 94Ā°C; 1' 52Ā°C; and 2' 72Ā°C) |
ESPB REV | 5' AAGCTTTAAACGTATCGACCATGAT | Ā |