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Table 3 Post mortem diagnosis in RLN of sheep with experimental BSE in preclinical and clinical status*.

From: Rapid and discriminatory diagnosis of scrapie and BSE in retro-pharyngeal lymph nodes of sheep

sheep nr.

m.p.i. at euthanasia

IHC†

WB‡

2117

6

-¶

2+

6869

9

+

1+

2114

12

+

2+

3903

13

+

2+

3907

15

+

2+

3905

17

+

2+

2112

19

+

2+

2140

21§

+

2+

2115

21§

+

2+

2138

23§

+

2+

2116

24§

+

2+

  1. * Oral inoculations occurred between 4–6 mo of age. Each animal received 5 g of bovine BSEinfected brain by oral route [27].
  2. † Positive in tonsil and RLN.
  3. ‡ 1+, weak signal for all three PrPres bands. 2+ PrPres bands clearly visible. Results after concentration obtained with either of the antibodies 9A2 or L42; antibody 12B2 scored negative in all samples.
  4. §Clinical signs.
  5. ¶ Weakly positive in tonsil and ileal Peyer's patches.