Skip to main content

Table 2 Sheep with preclinical scrapie*.

From: Rapid and discriminatory diagnosis of scrapie and BSE in retro-pharyngeal lymph nodes of sheep

   

diagnosis

sheep nr

genotype

age (m)

IHC

WB

   

brain

RLN†

brain‡

RLN‡

PS-5

VRQ/VRQ (n = 18)

2

-

1+

-

-

PS-6

 

2

-

1+

-

-

PS-3

 

3

-

2+

-

2+

PS-4

 

3

-

-

-

-

PS-1

 

4

-

4+

-

3+

PS-12

 

4

-

3+

-

2+

PS-7

 

5

-

4+

-

3+

PS-8

 

5

-

4+

-

1+

PS-10

 

5

-

4+

-

1+

PS-11

 

5

-

3+

-

2+

PS-18

 

10

1+§

4+

-

2+

PS-17

 

13

1+

4+

-

2+

PS-15

 

14

2+

4+

-

2+

PS-16

 

14

2+

4+

-

3+

PS-14

 

17

3+

4+

1+

2+

PS-13

 

21

3+

4+

3+

2+

PS-23

 

26¶

3+

4+

3+

2+

PS-24

 

26¶

3+

4+

3+

1+

PS-21

ARR/VRQ (n = 4)

17

-

-

-

-

PS-22

 

17

-

-

-

-

PS-19

 

24

-

-

-

-

PS-20

 

24

-

-

-

-

PS-2

ARR/ARR (n = 2)

3

-

-

-

-

PS-9

 

6

-

-

-

-

  1. * open flock of Texel cross breed with natural scrapie; – means no PrP or PrPres detected.
  2. † number of plus signs indicate % of follicles PrP-positive 1+ = <25%, 2+ = 25-50%, 3+ = 50–75%, 4+ = 75–100%.
  3. ‡ 1+ = weak signal for all three PrPres bands; 2+ = PrPres bands well visible; 3+ = PrPres bands so dark that diglycosyl and monoglycosyl are merging together at 6 min film exposure. Results obtained with either of the antibodies 12B2, P4 or 9A2 after SDS-PAGE of non-concentrated samples, or with 12B2 and concentrated samples.
  4. § In a previous study, another 10 m old RLN positive animal was found negative in the brain by IHC [25].
  5. ¶ Clinical signs.