Table 4 Nucleotide sequences of the primers and probes used in qPCR (CPV) and rt-qPCR (CDV; CCoV) assays

Forward 5’ → 3’

GGGCCTGGGAACAGTCTTGACC (900 nM)

TGGCACTCATTTTGGACATCAA (900 nM)

TGGTCATCGCGCTGTCTACT (900 nM)

Reverse 5’ → 3’

ACCAGAGCGAAGATAAGCAGCG (900 nM)

GCTAACCCAGCTTCCACAATGTA (900 nM)

AGGGTTGCTTGTACCTCCTATTACA (900 nM)

TaqMan® probe 5’ → 3’

FAM CGCCGCTGCAAAAGAACACGACGAAGC TAMRA (250 nM)

FAM TCCCCAGGGAACAAGCCTAGAATTGCT TAMRA (250 nM)

FAM TTGTACAGAATGGTAAGCAC TAMRA (250 nM)

Product

99 bp

100 bp

66 bp