Table 5 Specifications of oligonucleotides used for RT-qPCR 1-3
From: Is hepatic lipid metabolism of beef cattle influenced by breed and dietary silage level?
Gene symbol | Full gene name | Acc. Number1 | Primer pairs (5′-3′) | Amplicon length |
|---|---|---|---|---|
CPT1A | carnitine palmitoyltransferase 1A | XM_002699420.2 | F: TTCTTCTGGGGTCTACGATTCC | 119 |
R: GATGTGCTTGCTGTCCCTCAG | ||||
DGAT1 | diacylglycerol O-acyltransferase 1 | NM_174693.2 | F: TTGGCAGGTAAGGCGGC | 99 |
R: GGGGGCGAAGAGGAAGTAGT | ||||
ELOVL2 | fatty acid elongase 2 | NM_001083517.1 | F: GTCTTCTTACATGATGACGCTGGT | 72 |
R: ATTGGCTTTTTCCGGTATGTCTGA | ||||
ELOVL5 | fatty acid elongase 5 | NM_001046597.1 | F: CCCTCTCGGTTGGTTGTATTTC | 127 |
R: GTGGTCCTTTTGGTGCTCTCTC | ||||
FADS1 | fatty acid desaturase 1 | XM_002699285.2 | F: GTGGGTGGACTTGGCCTG | 103 |
R: TGGGGCTTGTCTTCATGGTC | ||||
FADS2 | fatty acid desaturase 2 | NM_001083444.1 | F: CGGCAAGAAGAAGCTGAAATACCTG | 92 |
R: CTGCTCATCCCTTTGTATTTCCA | ||||
FASN | fatty acid synthase | NM_001012669.1 | F: ATGGCGTTCCACTCCTACTTCA | 137 |
R: CTCTCCTGCCACTGGGTCTC | ||||
INSR | insulin receptor | XM_002688832.2 | F:ACGCTGGTGGTGATGGAGTT | 133 |
R: TCTCTGCCGCCATCTGAATC | ||||
PPARA | peroxisome proliferator-activated receptor alpha | NM_001034036.1 | F: CCAACAACAACCCGCCTTT | 125 |
R: CGTCTTCTCGGCCATACACA | ||||
SCD | stearoyl-CoA desaturase 9 | NM_173959.4 | F: CCATCAACCCCCGAGAGAAT | 76 |
R: AAGGTGTGGTGGTAGTTGTGGAA | ||||
SREBF1c | sterol regulatory element binding transcription factor 1 | NM_001113302.1 | F: ATCTCTTGGAGCGAGCACTGA | 115 |
R: AGGTACCCCAGGGCATCTG | ||||
RPS93 | ribosomal protein S9 | NM_001101152.1 | F: GAAGGTAATGCCCTGTTGCG | 141 |
R: CAGGCCCAGCTTGAAGACC | ||||
SDHA3 | succinate dehydrogenase complex subunit A | NM_174178.2 | F: TGCAGGAAGGCTGTGAGAAGAT | 100 |
R: GTCTCCACCAGGTCAGTGTTCC |