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Figure 2 | BMC Veterinary Research

Figure 2

From: A recombinant chimera comprising the R1 and R2 repeat regions of M. hyopneumoniae P97 and the N-terminal region of A. pleuropneumoniaeApxIII elicits immune responses

Figure 2

Expression and purification of ApxN, P97C, and Ap97 recombinant proteins. A. Coomassie blue-stained SDS-PAGE gel showing ApxN, P97C, and Ap97 purified from E. coli BL21 using His-tag affinity chromatography. The molecular masses of ApxN (lane 1), P97C (lane 2), and Ap97 (lane 3) were approximately 15, 45, and 60 kDa, respectively, which were equivalent to the expected values. B, C, and D. Western blot analysis of ApxN, P97C, and Ap97 using antisera from mice immunized with Ap97 (B), ApxN (C), or P97C (D) vaccines. The mouse antisera were obtained 3 weeks after the last immunization (Day 63).

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BMC Veterinary Research

ISSN: 1746-6148

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