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Figure 4 | BMC Veterinary Research

Figure 4

From: Biologic activity of the novel orally bioavailable selective inhibitor of nuclear export (SINE) KPT-335 against canine melanoma cell lines

Figure 4

Impact of KPT-335 on XPO1 message and protein expression in melanoma cell lines. A. Canine melanoma cell lines were treated with DMSO or KPT-335 1 μM for 24 hours and RNA was collected. Quantitative RT-PCR was performed for XPO1. Relative expression was found to be increased in treated cell lines with a p< 0.05. B. Canine melanoma cell lines were treated with DMSO or KPT-335 at 0.1 μM or 1 μM for 4 or 24 hours prior to collection. Protein lysates were generated, separated by SDS-PAGE and Western blotting for XPO1 and β-actin were performed.

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