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Figure 3 | BMC Veterinary Research

Figure 3

From: Biologic activity of the novel orally bioavailable selective inhibitor of nuclear export (SINE) KPT-335 against canine melanoma cell lines

Figure 3

Effect of KPT-335 on colony formation. Canine Mel 23 and Mel 36 cells were seeded at 2,000 cells per well in a 6 -well plate for 24 hours, followed by treatment with DMSO, 0.001, 0.01, 0.1, 1 or 10 μM KPT-335 for 7 days. Cells were then fixed and stained with crystal violet and colonies with greater than 50 cells were counted. The surviving cell fraction was defined as the number of colonies counted divided by the number of cells that were plated in the treated groups and then normalized to the plating efficiency. Plating efficiency was defined as the number of colonies divided by the number of cells plated in the untreated group. Vehicle control treated Mel 23 and Mel 36 cells served as the plating efficiency control group. The surviving fraction was significantly decreased in cells treated with > 10 nM concentration of KPT-335, p < 0.05.

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