Figure 4

Neural and multipotent gene expression profile in bMSC under differentiating conditions using neurogenic protocol 1. (A) bMSC were isolated by plastic adherence from bovine fetal BM and cultured under neurogenic or control conditions. Culture of fetal bMSC under neurogenic conditions induced formation of cell body retraction and formation of multiple and complex extensions since 24 h of culture. (B) Analysis of Q-PCR detected mRNA levels of neural markers NESTIN, MAP2, NGF and TRKA in fetal bMSC cultured under control (CON) and differentiating (DIF) conditions. No differences (P > 0.05) in mRNA levels were detected between control and differentiated cultures. (a,b) Superscripts represent significant (P < 0.05) differences between sampling hour. Bar scale: 500 μm.