Figure 2

Thin layer chromatography (TLC) (A) and immunoblot (B) of ethanol extracts of mycobacterial isolates . (A) Dried ethanol extracts of mycobacterial isolates were Folch washed and then developed on a silica-gel TLC plate using a mixture of Chloroform: Methanol: Water (90:10:1) to detect the presence of species specific molecules in the extract of M. bovis. The plates were sprayed with polymolybdate solution and the molecules were heated prior to visualization. Ethanol extracts of M. bovis, MAP (K10 Strain), MAP (Linda Strain) and M. avium subsp. avium were used in the lanes 1, 2, 3 and 4, respectively. (B) The MPB83 protein is present in the M. bovis ethanol extract. Proteins in M. bovis ethanol extract were separated by SDS-PAGE and transferred on to a nitrocellulose membrane. An anti-MPB83 monoclonal antibody labeled both the native MPB83 in the ethanol extract (lane 1, arrow) and the recombinant MPB83 fusion protein (lane 2, arrow).