Figure 1

A - Ethanol-vortex enzyme linked immunosorbent assay (EVELISA). Antibody binding on serum samples from uninfected (n = 40) and naturally infected (n = 22) cattle were tested by EVELISA test. All the uninfected animals were from a bovine TB free herd and the naturally infected animals were determined as infected by mycobacterial culture, histology and IS6110 PCR techniques. A cut-off value of 0.4 was used to distinguish M. bovis negatives and positive animals. B - EVELISA results from sera of deer: Antibody binding in serum of uninfected (n = 25), M. bovis -infected – naturally (n = 7) and experimentally (n = 9) deer was determined using the EVELISA test. A cut-off value of 0.068 was used to distinguish between M. bovis -negative and positive animals. Both the cut-off values were determined to maximize the sum of sensitivity and specificity values. All experiments were conducted in duplicate or triplicate and repeated at least twice. The statistical difference of antibody binding was evaluated using Mann–Whitney U test.