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Figure 3 | BMC Veterinary Research

Figure 3

From: Detection of total and PRRSV-specific antibodies in oral fluids collected with different rope types from PRRSV-vaccinated and experimentally infected pigs

Figure 3

PRRSV-specific IgG detection via immunoperoxidase monolayer assay (IPMA) and immunofluorescence assay (IFA). PRRSV-infected cells were incubated with 1:2 dilutions of oral fluid samples. PRRSV-specific IgG antibodies bound to infected cells were subsequently detected via IPMA (A), using biotinylated goat-anti-swine IgG antibodies and horseradish peroxidase-conjugated streptavidine, or IFA (B), using goat-anti-swine IgG antibodies and FITC-conjugated anti-goat antibodies.

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BMC Veterinary Research

ISSN: 1746-6148

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