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Figure 3 | BMC Veterinary Research

Figure 3

From: Rate of manual leukocyte differentials in dog, cat and horse blood samples using ADVIA 120 cytograms

Figure 3

Cytograms of the ADVIA 120 for horse samples. For remainder of key see Figure 1. (A) Cytograms without any abnormalities. (B) Suspicion of left shift: in the baso cytogram (B2), an indistinct separation between the mononuclear and polymorphonuclear cell populations is noted (“worm with swollen neck”). In the peroxidase cytogram (B1), few cells are scattered upwards from the neutrophil population indicating large and possibly toxic neutrophils. (C) No clear separation of cell populations and suspicion of left shift and myeloperoxidase deficiency of neutrophils. (D) Suspicion of atypical lymphocytes: in the peroxidase cytogram (D1), an increased number of cells are present in the LUC gate. In the baso cytogram (D2), few cells are scattered from the mononuclear area upwards into the lyse-resistant area. (E) Suspicion of blasts: in the peroxidase cytogram (E1), a large cell population extending from the lymphocyte gate into the LUC and monocytes gate indicating blast cells are present; in the baso cytogram (E2), a “blast nose” (arrow) can be identified and cells are scattered from the mononuclear area into the lyse-resistant area.

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