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Figure 1 | BMC Veterinary Research

Figure 1

From: Rate of manual leukocyte differentials in dog, cat and horse blood samples using ADVIA 120 cytograms

Figure 1

Cytograms of the ADVIA 120 for dog samples; Lym: lymphocytes; LUC: large unstained cells; Mon: monocytes; Neu: neutrophils; Eos: eosinophils; AGG: platelet aggregates; D: debris; MN: mononuclear cells; PMN: polymorphonuclear cells; Baso: basophils; Lyse-resistant: lyse-resistant cells. On the left-hand side, the peroxidase scattergrams (myeloperoxidase content on the x-axis and cell size on the y-axis) are depicted; on the right-hand side, baso cytograms (nuclear complexity on the x-axis and cell size on the y-axis) are shown. (A) Normal cytograms. (B) Suspicion of left shift: in the baso cytogram (B2), the mononuclear and polymorphonuclear cells are not well separated (“worm with swollen neck”). (C) No clear separation of cell populations: in the peroxidase cytogram (C1), neutrophils and eosinophils are not clearly separated. (D) No clear separation of cell populations and suspicion of reactive lymphocytes: in the peroxidase cytogram (D1), a separate cell population extending from the lymphocyte gate into the LUC gate is seen. (E) No clear separation of cell populations and suspicion of left shift: in the peroxidase cytogram (E1), the neutrophil population is shifted towards the upper left of the neutrophil gate, extending into the monocyte gate, which is indicative of myeloperoxidase deficiency of neutrophils. In the baso cytogram (E2), a shortened population of polymorphonuclear cells is present. (F) Suspicion of reactive lymphocytes: in the peroxidase cytogram (F1), a separate cell population extending from the lymphocyte gate into the LUC gate indicating atypical lymphocytes or blasts is seen; in the baso cytogram (F2), however, no major abnormalities are detected. (G) Suspicion of blasts: in the peroxidase cytogram (G1), a large cell population extending from the lymphocyte gate into the LUC gate indicating blast cells are present; in the baso cytogram (G2) a “blast nose” can be identified (arrow) and cells are scattering from the mononuclear area into the lyse-resistant area.

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